This step is used to download an MLST scheme (5-7 loci) from a public MLST database.

Choose your organism in the field on the top.
For most organisms there is only one MLST scheme listed. A preview is shown in the table at the bottom.

The 5' and 3' sequences are automatically extracted from the consensus of all allele libraries. The length can be defined in the fields.
The first allele sequence of each loci is used as reference sequence.

Press Next to proceed to the next step.

The list of available MLST scheme databases is taken from two databases:

• BIGSdb Oxford (PubMLST) - public databases for molecular typing and microbial genome of University of Oxford, Department of Zoology, Oxford, UK [1]
• BIGSdb Pasteur - the genomic-based strain taxonomy and nomenclature platform of Institut Pasteur, Paris, France [2]

Note the data access policy of both databases: since January 1st, 2025, registration and authentication are mandatory to access all data curated after this date.

An error message Authorized BIGSdb access failed, using access without authorization is displayed when the user is not authorised or not registered for the MLST database.

To access data that was submitted on 01.01.2025 or later use the the command Options | Account Details.